intraperitoneal injection in humans

The physiologic characteristic of the peritoneal cavity not only helps remove toxic metabolites from the body, but also provides a useful portal of entry in the body for several pharmacological agents. They are one of the few ways drugs can be administered through injection, and have uses in research involving animals, drug administration to treat ovarian cancers, and much more. Types of injections: Uses, sites, and what to expect - Medical News Today Google Scholar. Estes, J. et al. Rodents. All reactions were performed in triplicated. We believe with the increasing interests for IP injection in the field of cytotherapy, more innovation and creative ideas for reducing IP complications will come up. local inflammation and granulomatous reactions at the site of injection, lymph node structural changes, chronic inflammation, skin ulceration, local abscess or tissue sloughing, diffuse systemic . Rodent (large / mouse) oral (intragastric), intravascular administration (tail vein injection, intraperitoneal injection, intramuscular injection and subcutaneous injection) Intragastric administration and oral taking of non-rodent (dog / monkey) solution and intravenous injection of extremities. The IP technique is an easy technique to master. Cite this article. Best way for mesenchymal stem cells transplantation for colitis", "Intraperitoneal injections as an alternative method for micro-CT contrast enhanced detection of murine liver tumors", "Optimization of Intraperitoneal Injection Anesthesia in Mice: Drugs, Dosages, Adverse Effects, and Anesthesia Depth", "Intraperitoneal Injection Is Not Always a Suitable Alternative to Intravenous Injection for Radiotherapy", "Pharmacological principles of intraperitoneal and bidirectional chemotherapy", Transjugular intrahepatic portosystemic shunt, https://en.wikipedia.org/w/index.php?title=Intraperitoneal_injection&oldid=1142986299, Short description is different from Wikidata, Articles with unsourced statements from April 2015, Articles with unsourced statements from January 2022, Creative Commons Attribution-ShareAlike License 3.0, This page was last edited on 5 March 2023, at 09:43. Metabolite tracing, radioactive uptake experiments, and CRISPR screens demonstrate that FLVCR1 is the major plasma-membrane choline transporter. An improved mouse model of sepsis based on intraperitoneal injections of the enriched culture of cecum slurry . The fate of human peripheral blood lymphocytes after - PubMed PDF An improved mouse model of sepsis based on intraperitoneal injections While this route is an easy to master, quick, suitable for chronic treatments and with low impact of stress on laboratory rodents, there is a common concern that it may not be an acceptable route for drug administration in experimental studies. PDF EUTHANASIA REFERENCE MANUAL - HumanePro Intraperitoneal injection (IP), Intravenous injection (IV) or anal injection (AI)? In addition, the effects of different dosing modalities (e.g., oral administration and intraperitoneal injection) on the . If there is negative pressure, proceed with the injection depress the plunger until the solution has been fully administered. DiR-labeled cells were injected into DSS mice by three different ways. The bilateral femurs and tibias were aseptically excised, stripped of connective tissues and then stored in PBS. Journal of biomedical optics 11, 050507, doi: 10.1117/1.2364903 (2006). Adhesions are a leading cause of secondary infertility in women, accounting for 15% to 20% of cases. Solid lipid nanoparticle delivery of rhynchophylline enhanced the efficiency of allergic asthma treatment via the upregulation of suppressor of cytokine signaling 1 by repressing the p38 signaling pathway. (C) Percentage of body weight changes over time. Importantly, we identified different migration fates of MSCs and traced their ultimate location of three administration ways for the first time. Stem cell research & therapy 6, 55, doi: 10.1186/s13287-015-0066-5 (2015). Appendix E: Common Vehicles for Nonclinical Evaluation of Therapeutic PLGA Nanoparticles for the Intraperitoneal Administration of CBD in the Treatment of Ovarian Cancer: In Vitro and In Ovo Assessment. Further study demonstrated TSG-6 alone was sufficient to reduce intestinal inflammation in mice with colitis. In their study, PKH26 (a yellow-orange fluorescent dye) labeled PCs were injected intraperitoneally into healthy rat. query serum metabolite genome-wide association study (GWAS) data to identify an association between choline metabolism and feline leukemia virus subgroup C cellular receptor 1 (FLVCR1). Colitis showed varying degrees of alleviation after three ways of MSCs transplantation and the IP injection showed the highest survival rate of 87.5% and displayed the less weight loss and quick weight gain. Transplantability and therapeutic effects of bone marrow-derived mesenchymal cells in children with osteogenesis imperfecta. official website and that any information you provide is encrypted Gastrointestinal Absorption of Insulin-like Growth Factor If your cancer spreads to your abdomen or peritoneal space, IP chemotherapy can help target the cancer cells there. Wang Min, Liang Cong and Hu Hao contributed equally to this work. Scientific Reports (Sci Rep) Tuning the Physicochemical Characteristics of Particle-Based Carriers for Intraperitoneal Local Chemotherapy. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Biological Sciences, China Agricultural University, Yuanmingyuan West Road No. Whole marrow cells are plated in the culture dish and non-adherent cells were removed during passage. and transmitted securely. Shariati M, Zhang H, Van de Sande L, Descamps B, Vanhove C, Willaert W, Ceelen W, De Smedt SC, Remaut K. Pharm Res. In that same year, a study injected egg whites intraperitoneally into rats to study changes in "droplet" fractions in kidney cells. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Ankrum, J. J Vis Exp, doi: 10.3791/1488 (2010). However, more studies described MSCs as hit and run therapy, which discovered most of MSCs died and cleared from the body within 48 to 72h20,41,42,43. In 1996, intraperitoneal (IP) administration of cisplatin plus intravenous (IV) cyclophosphamide proved superior to both drugs given intravenously at the same doses-which, at the time, was the standard treatment in the United States. Select search scope, currently: articles+ all catalog, articles, website, & more in one search; catalog books, media & more in the Stanford Libraries' collections; articles+ journal articles & other e-resources However, most of these studies were focus on its therapeutic effects or mechanisms. Here, we also compare the expression of FoxP3, which is frequently used to quantitate Treg cells. PubMed These non-target entrapments made IV route less cells immigration and colonization and may be a hindrance for MSCs to fully display its therapeutic effects. Samples were prepared and tested as previously reported47. A . Intraperitoneal - an overview | ScienceDirect Topics PubMed PubMed Central Easier to inject that intravenous. Intraperitoneal injection (IP), Intravenous injection (IV) or anal injection (AI)? Though IP injection showed benefits in many human and animal . Intraperitoneal Drug Delivery for Ovarian Cancer: Why, How, Who, What Intraperitoneal injection or IP injection is the injection of a substance into the peritoneum (body cavity). Intraperitoneal Drug Administration - an overview | ScienceDirect Topics Vishnubalaji, R., Al-Nbaheen, M., Kadalmani, B., Aldahmash, A. [8], A good example of how intraperitoneal injections work is depicted through "The distribution of salicylate in mouse tissues after intraperitoneal injection" because it includes information on how a drug can travel to the blood, liver, brain, kidney, heart, spleen, diaphragm, and skeletal muscle once it has been injected intraperitoneally.[9]. 107vs. 109 ; Fig. Kyriakou, C., Rabin, N., Pizzey, A., Nathwani, A. This case presents the question of how THC is absorbed in the peritoneal cavity. Secondly, as mentioned above, there are plenty of immune cells in peritoneal cavity28, which are indispensable component of MSCs aggregates. Not only in animal studies, some clinical trials using MSCs to treat osteogensis imperfect (OI) in GvHD, also showed less than 1% cell were detected in target organ18,19,20. The measurement of inflamed colon length demonstrated therapeutic effects of MSCs injection. 2B). Stem cells and development 21, 21892203, doi: 10.1089/scd.2011.0674 (2012). Antunes, M. A. et al. IBD can be painful and persistent for life, which might decrease the quality of life. In IP group, cells were found accumulation at the epithelium; In AI group, cells were mainly in the lumen; In IV group, cells were fewer and located in lamina propria. NIR fluorescent dye, 1, 1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide (DiR; DiIC18(7), Molecular Probes, Invitrogen, Carlsbad, CA) was used for labeling the cells as previously reported65. These mice were then randomized into 3 groups receiving different MSCs giving therapies. Article However, one recent published paper showed inconsistent results with ours, in which IV injection was identified better in comparison with intraperitoneal injection31. In general, it is preferred when large amounts of blood replacement fluids are needed or when low blood pressure or other problems prevent the use of a suitable blood vessel for intravenous injection. Internet Explorer). If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. Fraguas-Snchez AI, Torres-Surez AI, Cohen M, Delie F, Bastida-Ruiz D, Yart L, Martin-Sabroso C, Fernndez-Carballido A. Pharmaceutics. Available at: http://ctep.cancer.gov/highlights/20060105_ovarian.htm (Accessed: 7th April 2016) (2006). government site. Qualitatively, the existence of non-portal drainage was demonstrated by . ADS You are using a browser version with limited support for CSS. The benefit of administering drugs intraperitoneally is the ability for the peritoneal cavity to absorb large amounts of a drug quickly. The complications associated with IP injection are major concern. Interaperitoneal Administration of lpha-Tocopherol Loaded Nanoparticles Improves Ischemia-Reperfusion Injury in Rat Ovaries Torsion and Detorsion Model. The medium was refreshed every 23 days and third- and fourth-passage cells were used for all experiments. The results of this IP injection were the disappearance of the ascites associated with the rupture. Endoscopic fluorescence imaging experiments were performed at 1-h postinjection of FA-NPs, celecoxib/FA-NPs or FNC-NPs using a Karl Storz-Endoskope System. Provided by the Springer Nature SharedIt content-sharing initiative. We have proposed that safe and effective protection against the development of adult onset cancers may be achieved by vaccination against tissue-specific self-proteins that are "retired" from expression at immunogenic levels in normal tissues as we age, but are overexpressed in emerging tumors. The most efficient method to inject small animals is a two-person method where one holds the rodent and the other person injects the rodent at about 10 to 20 degrees in mice and 20 to 45 degrees in rats. Figure 1. mRNA-engineered mesenchymal stem cells for targeted delivery of interleukin-10 to sites of inflammation. Ng, S. C. et al. PDF Chris Quigley Essentials Full Spectrum Curriculum (2023) In a case of a ruptured hepatocellular carcinoma, it was reported that the patient was treated successfully through the use of an intraperitoneal injection of OK-432, which is an immunomodulatory agent. Article Obermeier, F. et al. Stem cell transplantation showed promising results in IBD management. The fecal occult blood test on the day 3 also showed nearly complete absence of occult blood in IP group (Figure 1D). Gastroenterology 145, 158165 e152, doi: 10.1053/j.gastro.2013.04.007 (2013). With the computer-assisted software, we calculated the density of collagen fibers (%) of all groups. PDF Proper Method for Intraperitoneal Injections - PerkinElmer Best way for mesenchymal stem cells transplantation for colitis. Histological evaluation was completed in a double-blind fashion by two independent pathologist. PloS one 7, e33360, doi: 10.1371/journal.pone.0033360 (2012). 2020 Jun 3;37(6):119. doi: 10.1007/s11095-020-02818-8. Two early reports aroused the global interests of stem cell transplantation in IBD management. Action at a distance: systemically administered adult stem/progenitor cells (MSCs) reduce inflammatory damage to the cornea without engraftment and primarily by secretion of TNF-alpha stimulated gene/protein 6. . Course of Crohns disease after allogeneic marrow transplantation. and H.H. Intraperitoneal injection. For example, discharged accompany with stool or cleared out by lung microphage. & Dittel, B. N. Isolation of mouse peritoneal cavity cells. The GFP+ MSCs were found at inflamed colon in both IP and AI groups 24h after MSCs injection. Buy cheap Viagra Plus online no RX - Effective online Viagra Plus Epub 2010 Mar 3. Upon intravenous and intraperitoneal injection of stressed MSA, fluorescent "hotspots" were observed in the spleens, livers and lungs. There are few accounts of the use of intraperitoneal injections prior to 1970. Intraosseous. [20] There needs to be more research done to determine appropriate dosing and combinations of these drugs to advance intraperitoneal drug delivery. Your peritoneal space. . Quantification of collagen fiber was assessed using Image Pro Plus software (version 6.0). & Yong, K. Factors that influence short-term homing of human bone marrow-derived mesenchymal stem cells in a xenogeneic animal model. The natural history of adult Crohns disease in population-based cohorts. Haematologica 93, 14571465, doi: 10.3324/haematol.12553 (2008). Slow intravenous injections are typically dosed over 3-10 minutes. drug availability and human abuse potential; 10. compatibility with species, age, and health status; 11. doi: 10.1002/14651858.CD005284.pub3. In IV group, most of MSCs were trapped in the lung, liver and spleen, while the cell that immigrated to the colon was not too much. Fresh feces from animals were collected for occult blood test, using fecal occult blood kit (Baso Diagnostics Inc., Zhuhai, China) according to the manufactures recommendations. In anal injection group, same amount of cells were suspended in 200L diluted Matrigel in PBS (1:20) and the cell suspension was instilled into colonic lumen by a syringe with flexible catheter 4cm in length and 2mm in diameter, according to the previous study64. Given the range of potential doses, aiming for an injection volume of 5 l g -1 is advisable. In the meanwhile, the colonic homogenates were collected and colonic IL-10, TNF- were measured by ELISA assays. The primer sequences used were listed in Supplementary Table S1. The optimum route of administration is an importance clinical issue not only for chemical drug, but also for stem cytotherapy. intraarticular and intraperitoneal use of opioids, pain management in the emergency room and in the intensive care unit, pain management issues during pregnancy, geriatric pain, and hospice care and end-of-life issues. Their findings showed that intraperitoneally injected MSCs would form aggregates with macrophages and lymphocytes in peritoneal cavity and remotely secreted TSG-6. The authors give their special thanks to the Dr. Yingchun Jia and Zhen Chen for their help in histological evaluation. Another example is applying hammock catheter placement technique60. 2021. It is more often applied to animals than to humans. MSCs organ distribution and engraftment was analyzed and quantified by GFP+ MSCs as well as Near-infrared fluorescence imaging. Tests were read by an independent observer (Cong Liang) and scored 05 as shown by the color indicators provided by the manufacturer. PubMed Mice were made diabetic by intraperitoneal injection of streptozotocin, which led to a 2- to 2.5-fold increase in plasma glucose. In DSS control FoxP3 positive cells only accounted for 1.51% of total cells, while the IP group showed 13.5% positive cells (P<0.001). adult mice at 8-10 weeks of age were first anaesthetized by intraperitoneal injection of 20 mg/ml Avertin (Sigma-Aldrich) (15 l/g . gynecological and gastrointestinal cancers and has shown very promising results. Intraperitoneal Injection - an overview | ScienceDirect Topics Stem cells (Dayton, Ohio) 26, 279289, doi: 10.1634/stemcells.2007-0454 (2008). Hommes, D., Colombel, J. F., Emery, P., Greco, M. & Sandborn, W. J. iQ SYBR Green Supermix and CFX96 (Bio-Rad) were used for template amplification with a primer for each of the transcripts examined. In the guinea pig, the oral LD 50 value is 10 700 mg kg 1. A modified human recombinant type 5 adenovirus . Hsiao, S. T. et al. PubMed Rep. 6, 30696; doi: 10.1038/srep30696 (2016). For some chemotherapy, such as ovarian cancer, IP injection is used to attempt to localize the drug . Unable to load your collection due to an error, Unable to load your delegates due to an error. Thus, IP and AI routes are free of pulmonary embolism issue. Supplies: There is a little fluid in between the peritoneal lining and your organs. Gonzalez, M. A., Gonzalez-Rey, E., Rico, L., Buscher, D. & Delgado, M. Adipose-derived mesenchymal stem cells alleviate experimental colitis by inhibiting inflammatory and autoimmune responses. Intraperitoneal Drug Administration - ScienceDirect Small animals: 1 mL per 10 lb of body weight. To varying degrees, all the three MSCs delivery ways could ameliorate DSS-induced colitis. In a previous study, 14 mice were exposed to SWCNTs through intravenous (IV) injection to determine whether there were any adverse effects caused by SWCNTs on the mouse brain and also to find a safe application dosage if possible. Mouse insulin gradually decreased and was no more detectable 13 weeks after STZ injection. As mentioned above, a commonly used cell dose is 1106 cells/30g mouse, which would be equivalent to 33106/kg or approximately 2.3billion cells for a 70kg adult. 2014ZX09508002). The good cell-cell cross talk between MSCs and immune cells may be another contributor for better therapeutic effects. [10] The results depicted that ketobemidone consumption was significantly lower when patients controlled anesthetic through IP. The DiR dye alone was served as control. Cell stem cell 5, 5463, doi: 10.1016/j.stem.2009.05.003 (2009). The images or other third party material in this article are included in the articles Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. The three groups can decrease the mortality rate compared with PBS control, however, the IP injection showed the highest survival rate of 87.5% (P=0.0021vs. Control, Fig. Samples were frozen and thawed 3 times, centrifuged at 30,000g for 20minutes. Ko, I. K. et al. Distribution of GFP+ MSCs in colon 1 day after injection. Sphingosine-1-phosphate links persistent STAT3 activation, chronic intestinal inflammation and development of colitis-associated cancer. 2010 May;27(5):735-8. doi: 10.1007/s11095-009-0031-z. Indeed, Mariana et al. therapy cannot yet be considered as a standard of care for the first-line treatment of ovarian cancer: a systematic review", "Review of intraperitoneal injection of sodium pentobarbital as a method of euthanasia in laboratory rodents", "Changes in "droplet" fractions from rat kidney cells after intraperitoneal injection of egg white", "Errors Related to Different Techniques of Intraperitoneal Injection in Mice", "Postoperative pain after abdominal hysterectomy: a randomized, double-blind, controlled trial comparing continuous infusion vs patient-controlled intraperitoneal injection of local anaesthetic", "Cannabinoid Disposition After Human Intraperitoneal Use: An Insight Into Intraperitoneal Pharmacokinetic Properties in Metastatic Cancer", "Intraperitoneal Route of Drug Administration: Should it Be Used in Experimental Animal Studies? Another example of how intraperitoneal injections are used in studies involving rodents is the use of IP for micro-CT contrast enhanced detection of liver tumors. The https:// ensures that you are connecting to the & Correa, D. The MSC: an injury drugstore. Intramuscular. Clinical Ovarian and Other Gynecologic Cancer 5, 2426, doi: 10.1016/j.cloc.2011.08.001 (2012). Further control study comparing bowel signals on healthy mice and DSS mice after MSCs injection showed weaker NIR signal on healthy bowel in all three routes, which indicated fewer MSCs migration to the colon without colitis (Supplementary Figure S5). Influence of Aggregation and Route of Injection on the - PLOS (B) Quantification of FoxP3+ cells, the IP>IA and IV>DSS control, n=6 mice/group; n=3 for the control group. The incidence and impact of IBD is increasing worldwide. Treatment for peritoneal dialysis-associated peritonitis. Evid Based Complement Alternat Med. PubMed Google Scholar. Background. [citation needed], In humans, the method is widely used to administer chemotherapy drugs to treat some cancers, particularly ovarian cancer. They found different source of MSCs with different delivery routes exhibited distinct effects on lung and cardiovascular injury. [7] In 1969, errors depending on a variety of techniques of administering IP injections were analyzed, and a 12% error in placement was found when using a one-man procedure versus a 1.2% error when using a two-man procedure. Kean, T. J., Lin, P., Caplan, A. I. More recently the IP route has been used for chemotherapy in patients with intra-abdominal malignancies, i.e. Berberich, S., Forster, R. & Pabst, O. Additionally, the clinical translation is another concern. Furthermore, the body weight changes displayed the less weight loss and quick weight gain of IP injection group and the maximum group difference among was reached on day 3 (Fig. analyzed the data, J.L. New chapters on interventional . Histopathological alterations in the mouse brain were detected after . During the observation, the body weight changes and fecal blood and their survival date was faithfully record. This led to the patients being able to be discharged earlier than when anesthesia was administered continuously. It also shows how easily substances are absorbed through the peritoneal cavity after an IP injection. Lopez-Cubero, S. O., Sullivan, K. M. & McDonald, G. B. Peri/postnatal development in rats was impaired with reduced pup weights, increased stillbirths, and increased pup mortality concurrent with maternal toxicity after intraperitoneal administration of tazobactam alone at doses 320 mg/kg/day (2 times the human dose based on body surface area) or of the combination piperacillin and tazobactam at . All fluorescent imaging procedures were conducted on small animal imaging system (IVIS Kinetics; Caliper Life Science). DailyMed - PIPERACILLIN AND TAZOBACTAM injection, powder, lyophilized Injections, also known as shots, deliver liquid medications, fluids, or nutrients directly into a person's body. Zhu, H. et al. The ELISA kit for TNFinduced protein 6 was used (CUSABIO Life science, China). Hepatic and renal . Summers, R. W. Novel and future medical management of inflammatory bowel disease. Proper Method for Intraperitoneal Injections In vivo clearance of free DiR in healthy mice was showed in Supplemental Figure S4. Intraperitoneal injections are a way to administer therapeutics and drugs through a peritoneal route (body cavity). Arthritis and rheumatism 56, 11751186, doi: 10.1002/art.22511 (2007). These two kinds of MSCs differ in proliferation rates and differentiation capacities as well as show significant difference in cytokine secretome and chemokine receptor expression which may affect migration, engraft and even local function32,33,34,35,36. PubMed Central Disease recovery was evaluated by histological server score, gross body weight and survival rate. Intraperitoneal injection (IP), Intravenous injection (IV) or anal injection (AI)? Kurtz, A. Mesenchymal stem cell delivery routes and fate. The peritoneum is a cavity which has been successfully utilized by nephrologists to perform peritoneal dialysis (PD) in patients with renal failure. Comparison of colonic FoxP3 expression 7 days after MSCs injection. The IV group showed 20.3% ki-67+ cells/crypt. A., Ong, J. F. & Karp, J. M. Mesenchymal stem cells: immune evasive, not immune privileged. completed the main experiment and wrote the first draft of the paper. Mesenchymal stem cells induce mature dendritic cells into a novel Jagged-2-dependent regulatory dendritic cell population. ***P<0.001; **P<0.01. Some of the examples depict situations where IP injections are not ideal, while others prove the advantageous uses if this delivery method. GAPDH mRNA was used as an internal control to normalize mRNA expression. The Ki-67+ cells were greatly increased after MSCs treatment. Researchers has tried many creative ways to minimize these risks. They are mitomycin C, cisplatin, carboplatin, oxaliplatin, irinotecan, 5-fluorouracil, gemcitabine, paclitaxel, docetaxel, doxorubicin, premetrexed, and melphalan. Understanding when intraperitoneal injections can be utilized and in what applications is beneficial to advance current drug delivery methods and provide avenues for further research. Intraperitoneal injection is not always a suitable alternative to Castelo-Branco, M. T. et al. After antigen retrieval, the endogenous peroxidase activity was blocked by 1% H2O2 for 10min. Comparison of molecular profiles of human mesenchymal stem cells derived from bone marrow, umbilical cord blood, placenta and adipose tissue. Fluorescent intensity was quantified and processed using the Living Image Software (Version 4.2; Caliper Life Science). Sign up for the Nature Briefing newsletter what matters in science, free to your inbox daily. [2] Intraperitoneal injections can be similar to oral administration in that hepatic metabolism could occur in both. https://doi.org/10.1038/srep30696. Bull Emerg Trauma. of the needle exposed to assure 4-5mm injection depth. and K.W. Federal law restricts this drug to use by or on the order of a licensed veterinarian. Generally, the mice were killed by cervical dislocation. The provided examples show a variety of uses for intraperitoneal injections in animals for in vitro studies. Murine Responses to Endotoxin: Another Dirty Little Secret? Journal of immunology (Baltimore, Md. Development of Pluoronic nanoparticles of fluorocoxib A for endoscopic was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity . Article Article showed that incubation of human monoclonal IgG aggregates in plasma for 24 hrs resulted in alteration of the total number of aggregates, led to different aggregate size and changed their . seem to have found the key drug of MSCs in the treatment of colitis, TSG-647. Here, we combined the iDISCO + method and Fos immunostaining to confirm that the middle part of the right-side, but not the left-side, insular cortex in adult male mice is activated by intraperitoneal injection of lithium chloride. & Ramesh, T. Comparative investigation of the differentiation capability of bone-marrow- and adipose-derived mesenchymal stem cells by qualitative and quantitative analysis. At 72h, the IP groups showed significant higher level of TSG-6 than AI groups and DSS control (P=0.023 and P=0.005, respectively). In IV group, the colon homing cells were fewer, most of MSCs were trapped in MSCs were rapidly accumulate into the lung and mononuclear phagocytic system.

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